Technical Summary Download PDF
  • : M15019
  • : Lactobacillus Ferment
  • : Approved
  • : Fully Compliant
  • : 68333-16-4
  • : N/A
  • : Biotechnology: Lactobacillus
  • : GMO Free, No Ethoxylation, No Irradiation, No Sulphonation, No Ethylene Oxide treatment, No Hydrogenation
  • : None
  • : None
  • : None
  • : None
  • : Water
  • : Clear to Hazy Liquid
  • : Aqueous Ferment Filtrate
  • : 2.0 - 4.0%
  • : Skin Conditioning, Antimicrobial

Leucidal® Liquid SF


Consumer choice is the most important factor when it comes to cosmetic sales. Today, a growing number of consumers are opting to move away from synthetic preservatives such as parabens, formaldehyde donors and phenoxyethanol. In addition to public pressure, the use of these synthetic materials in cosmetics is also becoming more strictly regulated. For these reasons, formulators have been actively searching for alternatives to synthetic preservatives that can provide broad spectrum antimicrobial activity and can be added into a wide range of cosmetic applications.

Active Micro Technologies (AMT) has developed a full line of products derived from naturally occurring compounds that provide broad spectrum antimicrobial protection. As a result, these novel natural antimicrobials are considered self preserving cosmetic actives and therefore can be used as consumer-friendly alternatives to synthetic preservatives in a wide range of cosmetic applications.


Leucidal® Liquid SF is a probiotic based ingredient created by the fermentation of Lactobacillus in a defined growth medium. Lactobacillus is one of the species of microorganisms used to produce fermented products such as sauerkraut and kimchi, a Korean dietary staple, from cabbage. Like many members of the lactic acid bacteria family, Lactobacillus is capable of restricting the growth of other microorganisms by acidifying its environment. However, in addition to acidifying its environment, Lactobacillus also produces novel antimicrobial peptides, also known as bacteriocins, that are capable of providing broad spectrum antimicrobial protection. During the manufacturing process, lysozyme is added to the ferment filtrate to facilitate a controlled cell lysis. This step helps ensure the release of the antimicrobial peptides for maximized activity.

M15019-Leucdial Liquid SF-MIC Test-v1-01Benefits

The ability of Leucidal® Liquid SF to inhibit the growth of a variety of bacteria and fungi was determined using the Minimum Inhibitory Concentration (MIC) test. The results show that this material provides broad spectrum antimicrobial protection.

A double challenge test using 2% Leucidal® Liquid SF was also conducted to evaluate the ability of the product to provide antimicrobial protection in cosmetic finished products. A basic O/W emulsion was used as the base. The samples were inoculated with E. coli, P. aeruginosa, S. aureus, C. albicans and A. brasiliensis and incubated for 28 days. During this period, samples were periodically collected and tested for the presence of viable microorganisms. Following the initial 28 days of incubation, the samples were reinoculated with the microbial cultures for another period of 28 days.

Use Recommendations

Leucidal® Liquid SF can be used in a wide range of cosmetic products, however to ensure optimum results we recommend adding it into products with a pH between 3 and 8 and during the cooling phase of the formulation process at temperatures lower than 70°C.

Challenge Tests

Cosmetic Efficacy