Technical Summary Download PDF
  • : M14005
  • : Lactobacillus Ferment Lysate
  • : Conforms
  • : Compliant
  • : 68333-16-4 [AICS, Revised ICL] (or) 92128-79-5 [PICCS]
  • : N/A (or) 295-777-8
  • : Biotechnology Lactobacillus
  • : GMO Free, No Ethoxylation, No Irradiation, No Sulphonation, No Ethylene Oxide treatment, No Hydrogenation
  • : None
  • : None
  • : None
  • : None
  • : Water
  • : Clear to Slightly Hazy, Colorless to Yellow Liquid
  • : Water Soluble
  • : 2.0 - 4.0%
  • : Antimicrobial, Redness Reduction, Scalp Care

ProBiocin V™


Scalp care is a prevalent branch of hair care, as a healthy scalp means healthy hair growth. This knowledge drives consumer’s interests in products that can restore balance to an “unhealthy” scalp. Malassezia spp. has been shown to play a key role in the pathogenesis of dandruff. Inspired by dandruff related irritation, research has driven product development to address Malassezia and balance the scalp microbiome.

The culturing of the probiotic bacterium Lactobacillus acidophilus, supported by prebiotic nutrients, diminishes the presence of Malassezia on the scalp, while presenting a new global approach to non-traditional preservative technologies. ProBiocin V™ harnesses the benefits of prebiotic oligosaccharides to offer a competitive edge to commensal microorganisms in their natural ecosystem. This optimized production method of supplementing the growth media aids in the production of postbiotic bacteriocin peptides, capable of delivering moisturizing and redness reduction benefits to soothe an irritated scalp.

Current efforts to reduce the prevalence of microorganisms associated with dandruff, involve the development of scalp and hair care products able to alleviate caustic pathogenic microorganisms, while relieving the scalp from itchiness. Traditional preservatives, frequently used in hair care applications, have been shown to be detrimental to both commensal and pathogenic bacteria living on the skin and scalp.

ProBiocin V™ is a technology able to diminish concerns associated with the use of synthetic preservative systems, while providing efficacious benefits against scalp irritation. The produced bacteriocin peptides are also capable of providing broad spectrum antimicrobial protection in order to maintain the integrity of the formulation. ProBiocin V™ acts as a multifunctional system that boosts preservation while delivering soothing benefits to the scalp. ProBiocin V™ is also vegan and compliant in many global markets.

Veganism is a popular trend in the cosmetic industry as well. As consumers are making lifestyle choices that make them feel healthier, the same logic applies to the products they use. Although you do not have to be vegan to use vegan products, consumers are beginning to pay more attention to what goes into the products they use and how these products will benefit them long-term. While shifting towards animal alternatives and away from ethical concerns, sacrificing the quality of ingredients is not an option when it comes to treating common cosmetic concerns such as moisturization and redness.


Prebiotic chicory oligosaccharide fructans are supplemented into the Lactobacillus growth media during fermentation. These bacteria produce postbiotic antimicrobial peptides (bacteriocins). Lysozyme is then used to facilitate controlled cell lysis of the ferment filtrate. When applied to the scalp, beneficial microorganisms are enriched and the microbiota is modulated, in effort to achieve a homeostatic environment. The Lactobacillus derived peptides work to condition the scalp, while reducing pathogenic microorganisms to soothe irritation.


Acting as a multifunctional system that boosts preservation while delivering soothing benefits to the scalp, ProBiocin V™ utilizes the properties of prebiotic oligosaccharides as an approach to postbiotic bacteriocin procurement to deliver scalp moisturization and redness reduction.

The ability of ProBiocin V™ to inhibit the growth of a variety of bacteria and fungi was determined using the Minimum Inhibitory Concentration (MIC) test. The results demonstrate that this material provides broad spectrum antimicrobial protection.

Use Recommendations

ProBiocin V™ can be used in a wide range of cosmetic products, however to ensure optimum results we recommend using the following guidelines. Incorporate the product into formulations at a pH between 3 and 8, during the cooling phase of the process at temperatures lower than 40°C.

Challenge Tests

Cosmetic Efficacy